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The following statements describe SDS-PAGE, a method used to separate proteins. Some of the statements are correct, and some of them are incorrect. Select all the correct statements.
Statements:
-SDS-PAGE uses a polyacrylamide gel to separate proteins.
-Sodium dodecyl sulfate denatures the proteins, resulting in protein separation by charge only.
-Smaller proteins migrate farther than larger proteins
-In SDS-PAGE, the more negatively charged proteins elute first from the column.
-Sodium dodecyl sulfate dentures proteins, resulting in proteins that are the same shape. (Assume beta-mercaptoethanol is also present to reduce disulfide bonds.)
-In SDS-PAGE, proteins migrate toward the negative electrode.
The group of molecular biologists is trying to synthesize a new artificial compound to mimic the effects of a known hormone that influences sexual behaviour. They have turned to you for advice.
Determine the significance of the observation that knocking out MyD88 abrogates increases in Factor B and C3a but knocking out of TRIF does not ? Describe in molecular terms.
A storm brings two formerly separated populations of beetles together. They look very similar. Under the biological species concept, which would show that the two populations are different species.
What is the difference between heterosporous and homosporous plants? What two types of spores do heterosporous plants produce? Spores that will develop into a female gametophyte are.
why the sequences are read in different reading frames.
Your friend is trying to generate an in vitro system for studying the formation of COPII vesicles. He finds that to get well-organized vesicle budding from artificial membranes, he needs to add the coat proteins, the Sar1 GTP-binding protein.
What have to be true of the cell wall of this bacterium? Mammalian blood contains an equivalent of 0.15 M. Seawater contains the equal of 0.45 M. What will occur if red blood cells are transferred to seawater.
What was this experiment and why was it so significant? Diagram and briefly describe the steps of the process. How and why has recombinant DNA technology impacted biotechnology.
He was puzzled, combative, and he refuses to drink any water or any liquid. He lived on Oct. 7th, 1849. While said to the man's name, the student thought "Nevermore!" What was his diagnosis.
If I had a biosphere of plants and animals and air and I radio labelled the carbon in co2, what path will that carbon take through the biosphere, where does carbon go.
Swelling of the abdomen and legs due to edema is characteristic of starving children with kwashiorkor, protein-deficiency disorder. What is the relationship between this symptom and procedure of capillary exchange.
Diplomonads, such as Giardia contain two haploid nuclei per trophozoite. Thus, During the G1 phase of the cell cycle, there should be a total of how several unreplicated chromosomes per trophozoite, and throughout the G2 phase, how many replicated ch..
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