Describe ph and biochemistry

Reference no: EM13132632

PH and Biochemistry

Determine the weight in grams of sodium dihydrogen phosphate (M.W.138.01) and disodium hydrogen phosphate (M.W 141.98) needed to prepare 1L of a standard buffer at pH 7.00, with a total phosphate concentration of 0.100M.

Okay I know that the pKa is going to be 6.86. I am therefore going to take the difference of pH 7 and pH 6.86 to get 0.14. I then do 10^(14) to get 1.38. However, I am lost after that step. I cannot seem to figure out the concentration. Once I know that, I can find the mass of each of the molecules.

Reference no: EM13132632

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