Describe how you would go about isolating mutations

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Question: You want to find genes involved in thiamine biosynthesis in a new bacterium you just discovered. You know how thiamine is synthesized in bacteria like E. coli and Salmonella. Using what is known from these strains (pathway is shown above), describe how you would go about isolating mutations in all genes that could lead to a thiamine auxotrophy?

Reference no: EM133550159

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