Describe how you can heat-fix slides

Reference no: EM13335882

1. What is the total magnification of microscope when you use a 10x ocular lens (eyepiece) and a 100x objective ? ( 2 pts)

2. Describe how you can “heat-fix” slides ( 2 pts):

3. Name one dye that can be used for non-specific staining of bacteria (2pts)

4. Outline the gram stain procedure and indicate what color is positive and what is negative for the outcome: (5 pts)

5. In the procedure of gram-stain, you have forgotten to use acetone alcohol, what color would Pseudomonas aeruginosa be? (2 pts)

6. Distinguish between Staphylococci and Streptococci and name a place where each bacterium may be found. (3 pts)

7. Discuss how you can differentiate between Staphylococcus auerus and Staphylococcus epidermidis. (3 pts)

8. What is the dye and sugar present in MSA agar and why would it turn yellow after incubation with Bacillus subtilitis or Staphylococcus auerues ? (2 pts)

9. Name one bacteria produces green metallic sheen on EMB plates. Explain why such colonies are formed. (2 pts)

10. Describe the catalse test, state the result for a positive reaction and write a balanced chemical equation to explain for the observed phenomonon: (3 pts)

11. Name one media that will only allow Gram positive bacteria, but not Gram negative bacteria to grow. (2 pts)

12. Which of the following is gram negative, produces a blue green pigment on TSA plate, and liquefies gelatin ? (2 pts)

A. Staphylococcus aureus

B. Staphylococcus epidermidis

C. Escherichia coli

D. Proteus vulgaris

E. Pseudomonas aeruginosa

13. Name one motile bacteria and a test to test for motility: (2 pts)

14. Which of the following organism is the most resistant to antimicrobial agents? (2 pts)

A. Pseudomonas aeruginosa

B. Streptococcus pneumoniae

C. Enterococcus faecalis

D. Escherichia coli

E. Stapylococcus aureus

15. Name the unit used in measuring the diameter of clear zone in a disk diffusion test of antimicrobial agents: _______ ( 2 pts)

16. Name one bacteria that can reduce nitrate all the way to nitrogen gas (2pts)

17. Which of the following ferments lactose? (2 pts)

A. Escherichia coli

B. Streptococcus pneumoniae

C. Streptococcus viridans

D. Streptococcus pyogenes

E. Pseudomonas aeruginosa

18. Bacteriophages that infect bacteria forms a clear zone on a lawn of bacteria known as: ______________ (2 pts)

19. An acid fast-positive bacterium such as Mycobacterium would appear __________ color after the acid fast stain ( 2pts)

20. Name one bacteria that would produce endospores (genus and species name required) ______________________ (2 pts)

21. Name the disinfectant solution used in the lab: (2 pts)

22. Which of the following reagent is the MOST effective as a hand antiseptic? (2 pts)

A. alcohol

B. windex

C. mouthwash

D. antibacterial soap

E. hydrogen peroxide

23. At what temperature are bacteria normally incubated? ____?C (2 pts)

24. Name the dye that is added to a fermentation tubes for fermentation tests: (2 pts)

25. Why would the color turn yellow for a positive fermentation test? (2 pts)

26. Name one anaerobic organism that can only grow in the absence of oxygen (genus and species name required) (2 pts)

27. After performing a serial dilution, you obtain 80 colonies on the plate after diluting 1 million times, 1 ml was plated onto the plate, what is the original number of bacteria in the culture ? (2pts)

A. 80

B. 8 million

C. 80 million

D. 800 million

E. cannot be determined.

Reference no: EM13335882

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