Describe how hpaii and mspi can be used to determine

Reference no: EM133393923

Methylation Analysis Assignment Instructions

Overview

You were introduced to several methodologies for determining DNA sequence and methylation states. This assignment will assess your ability to construct a logical essay-style response concerning these methods. It will also assess your ability to interpret the findings presented in the assigned research article in the Learn section (Read: Amyloid-β Alters the DNA Methylation Status of Cell-fate Genes in an Alzheimer's Disease Model).

Instructions

Compose a paragraph-style response for one of the essay topics listed below. The assignment must reflect your own understanding of the course material

Describe how HpaII and MspI can be used to determine if a given DNA sequence is methylated. Be sure to mention positive and negative controls and any limitations associated with these enzymes.
How does bisulfite treatment coupled with PCR enable the identification of DNA methylation?
How could a Southern blot be used to determine if a given DNA sequence contains methylated CpGs?
How does Figure 6 in the research article referenced above support the model proposed in Figure 7?

Reference no: EM133393923

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