Describe how hpaii and mspi can be used

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Reference no: EM133771567

Compose a paragraph-style response for one of the essay topics listed below. The assignment must reflect your own understanding of the course material and, as such, be written in your own words.

Question 1. Describe how HpaII and MspI can be used to determine if a given DNA sequence is methylated. Be sure to mention positive and negative controls and any limitations associated with these enzymes.

Question 2. How does bisulfite treatment coupled with PCR enable the identification of DNA methylation?

Question 3. How could a Southern blot be used to determine if a given DNA sequence contains methylated CpGs?

Question 4. How does Figure 6 in the research article referenced above support the model proposed in Figure 7?

Reference no: EM133771567

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