Describe how crispr was firstly discovered

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Questions:

Describe how CRISPR was firstly discovered. Explain CRISPR/Cas9 gene editing mechanism in microbial system.

List 2 examples of applications of CRISPR/Cas9. What are the potential problems for CRISPR/Cas9 clinical application? How to improve?

You are assigned to perform knockout an oncogene Kras gene in a prostate cancer cell line DU145 using CrispR/Cas, list the steps to accomplish this task, including the oligo sequences, vectors, transfections, controls, screening and verification.

What is the most promising CRISPR application in your mind? Why? How Doudna thinks (using her recent interview)?

Reference no: EM133340542

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Describe how crispr was firstly discovered : Describe how CRISPR was firstly discovered. Explain CRISPR/Cas9 gene editing mechanism in microbial system. List 2 examples of applications of CRISPR/Cas9.

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