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Questions:
Describe how CRISPR was firstly discovered. Explain CRISPR/Cas9 gene editing mechanism in microbial system.
List 2 examples of applications of CRISPR/Cas9. What are the potential problems for CRISPR/Cas9 clinical application? How to improve?
You are assigned to perform knockout an oncogene Kras gene in a prostate cancer cell line DU145 using CrispR/Cas, list the steps to accomplish this task, including the oligo sequences, vectors, transfections, controls, screening and verification.
What is the most promising CRISPR application in your mind? Why? How Doudna thinks (using her recent interview)?
In the cross MMBb x Mmbb, what is the probability of a foal having a long mane and green eyes, or, a long mane and brown eyes?
Discuss the regulation, phosphorylation, and activity of glycogen phosphorylase and glycogen synthase in terms of insulin and glucagon. What are the differences between glycogenolysis in the liver and in the muscle?
Explain the differences between positive and normative economics? Provide two examples of each after each of your responses.
Consider God's design for the body of believers in Christ. Provide examples of how body levels are interconnected.
Describe the treatment process used to clean wastewater in order for it to be allowed back into the water cycle or reused.
How and why does muscle contraction, increase in enzyme activity (LDH) and SNS activation contribute to lactate production during exercise? Cite each of the sources used.
What is purpose of the Gram's iodine?
Explain genetic engineering of transgenic plants and give an example.
Illustrate the environmental conditions that would favour sexual reproduction over asexual reproduction in Hydra. What is the possible evolutionary reason for having two different reproductive strategies.
"Discuss the possible presenting symptoms after a hard knock on the leg in the area behind the knee?"
The flipping of Phe rings requires that groups surrounding the ring move aside momentarily. The rate of ring flipping can be measured using NMR.
1. Describe what would happen if all of your skeletal muscles contracted at the same time. 2. Describe the two separate metabolic needs that are met when a full-grown person eats a balanced diet.
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