Reference no: EM132976801

Question 1.

Below are the first fifty nucleotides in the sequence of the mouse fetal beta globin epsilon gene. You are interested in how the fetal beta globin epsilon gene is expressed; spatially, temporally and/or constitutively. Design and describe an experiment making use of the provided nucleotide sequence to analyze fetal beta globin epsilon gene expression. (Sequence provided is the nucleotide sense strand from Genbank).

5'-gacaacctca agtctgcctt ggccaagctc agtgaactgc actgtgacaa-3'

Question 2.

You've been provided with the complete nucleotide sequence for the domestic dog pancreatic amylase AMY2B gene. Explain how you would search for and locate a homologous gene in the Fennec fox, assuming it has had its genome sequenced. Describe how you would obtain the original amylase protein, from each test specimen without obtaining it from the dog or Fennec fox directly (no blood, tissue or serum draws), and test it for comparative activity between the domestic dog and the Fennec fox. (Note, no field work or hands-on work with live dogs or Fennec foxes).

Question 3.

You're working on a newly discovered gene and are tasked to quantitatively quantify the precise transcript levels of this gene being expressed in a culture of cells. Explain the method you will use and details of how you will perform this.

Question 4.

You have purified a novel protein and have a small aliquot of it. Unfortunately, there's no commercially available antibodies to this new novel protein of yours. What can you do to obtain monoclonal antibodies to your new, novel protein so that you can use an antibody to screen sets of tissue samples to determine if your protein is expressed? You also want to have antibodies to use for a later time for other possible experiments.