Describe a mechanism for cytoplasmic retention

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Question 1.

Bacterial cells couple the process of transcription and translation. In eukaryotic cells, the process of transcription and translation are uncoupled or occur separately. Provide explanations as to why bacterial cells can couple the process of transcription and translation while in eukaryotic cells, the process is separated.

Question 2.

You're growing a culture of E. coli cells without glucose that has a mutation in the lac operator sequence such that it can't bind the lac repressor. Explain what would be occurring to the Lac operon in the presence of lactose. Explain what would be occurring in the absence of lactose.

Question 3.

You're studying a new protein and suspect that it may be a new type of transcription factor. You clone its sequence into a plasmid vector with a fluorescent tag so you can visualize its location. You observe that the new protein spends part of the time in the cell cytoplasm and part of the time in the cell nucleus. Describe a mechanism for its cytoplasmic retention and subsequent signal-mediated import. Design an experiment to test your hypothesis.

Question 4.

What is the histone code and describe what evidence there is both for and against this covalent modification of chromatin?

Reference no: EM132979034

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