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A new protein of unknown structure has been purified. Gel filtration chromatography reveals that the native protein has a molecular weight of 240,000. Chromatography in the presence of 6 M guanidine hydrochloride yields only a peak for a protein of Mr 60,000. Chromatography in the presence of 6 M guanidine hydrochloride and 10mM 2-mercaptoethanol yields peaks of Mr 34000 and 26000. Explain what can be determined about the structure of this protein from this data.
The amount of lead in a aqueous sample is determined by atomic absorption spectrometry. Two standard solutions are prepared and used to make a calibration curve, and then the absorbance of the sample is measured.
A robust first grader goes for swimming lessons three times a week, and he enjoys it thoroughly. During the first week of December, he develops an upper respiratory infection, starts coughing continuously, and has a high fever of 39°C.
What will be the resultant pressure when the stopcock is opened?
Sodium bicarbonate is often used as an antacid to neutralize excess hydrochloric acid in an upset stomach. What mass of hydrochloric acid (in grams) can be neutralized by 2.3g of sodium bicarbonate?
When 3.00 mol of benzene is vaporized at a constant pressure of 1.00 atm and at its normal boiling point of 80.1 degreesC, 101.8 kJ are absorbed and the P(delta)V for the vaporization process is equal to 8.70 kJ then
Under what conditions can you ensure that substitution will predominate and under what conditions can you ensure that elimination will predominate?
In the preparation of the esters given in this experiment, the reaction product was extracted with 5% sodium bicarbonate solution in the isolation step. Why? What gas was evolved during this washing step? Write a balanced equation for the reaction..
What are the correct oxidation states for the following compounds? P in H3PO4, Mn in Mn(O4)2- anion, and O in O2F2
A student wants to make a pH 4.0 buffer solution. She decides to use a buffer made from formic acid and sodium formate (NaCOOH).
A metal sample weighing 71.9 g and at a temperature of 100.0 degrees C was placed in 41.0 g of water in a calorimeter at 24.5 degrees C. AT equilibrium the temperature of the water and metal was 35.0 degrees C. What was delta t for the water?
Which of the experimental results for the photoelectric effects suggest that light can display particle-like behavior
What is the theoretical yield in grams? What is the theoretical yield in moles? (I already have the calculated amounts of reactants) Just need theoretical yields.
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