Define use of expression vector to isolate gene from a cdna

Assignment Help Biology
Reference no: EM13935277

1. Describe DNA sequencing with reference to the cloning of a DNA fragment in a bacteriophage M13 based cloning vector.

2. Describe the use of a expression vector to isolate the gene from a cDNA library that codes for a specific protein.

Reference no: EM13935277

Questions Cloud

Is the company approach to boosting profit ethica : Is the company's approach to boosting profit ethical?
Ben ren and vectra rey are talking to their friend bb king : Ben Ren and Vectra Rey are talking to their friend BB King are sitting at rest on a bench in the park feeding pigeons. Ben and Vectra are arguing about the forces on BB. Ben says, "Clearly BB is simply sitting there doing nothing as usual. Therefore ..
Prepare the consolidated balance sheet of razorback express : Razorback Express, Inc., paid $266,000 to acquire all the common stock of Sooner Corporation, and Sooner owes Razorback $81,000 on a note payable. Prepare the consolidated balance sheet of Razorback Express
Find out about desktop virtualisation : The management of your employer wants to find out about desktop Virtualisation and how it works. They think that there may be some advantage to the organisation from adopting some of these desktop Virtualisation technologies.
Define use of expression vector to isolate gene from a cdna : Describe DNA sequencing with reference to the cloning of a DNA fragment in a bacteriophage M13 based cloning vector.
Determine the compressive force and the shear force : Determine the compressive force C and the shear force S supported by this disk in terms of the body weight W. The weight Wu of the upper torso (above the disk in question) is 68% of the total body weight W and acts at G1.
What are the four steps involved in the preparation : What are the four steps involved in the preparation of a production cost report?
Explain the viral strategy : The promoters for mRNA encoding early proteins in viruses like T4 have a different sequence than the promoters for mRNA encoding late proteins in the same virus. Explain the viral strategy behind this and how promoter modification occurs.
Explain the idea behind the percentage-of-completion method : Draft a letter to Sue, telling her about the percentage-of-completion method of recognizing revenue. Compare it to the completed-contract method. Explain the idea behind the percentage-of-completion method

Reviews

Write a Review

Biology Questions & Answers

  Q1 how many nucleotides would it take to construct the

q1. how many nucleotides would it take to construct the coding strand of the globin molecule? explain how you

  Choose one plant from the lists write a page essay

Choose one plant from the lists below and write a page essay. Give the common name, scientific name, life cycle, and characteristics.

  How many times more hydroxide ions are there in a sloution

How many times more hydroxide ions are there in a sloution with a pH of 9 then in a solution with a pH of 3.

  Explain the pattern of base pairing

Estimate what type of bond joins one strand of a DNA duplex to the other strand and what term is used to describe the pattern of base pairing between one DNA strand and its partner in a duplex?

  Difference between interspecies and intraspecies competition

Explain the difference between interspecies and intraspecies competition. Provide an example of each: interspecies and intraspecies competition. List the reasons a population reaches its carrying capacity

  Using chlorophyta as the out group-bryophyta-anthophyta

Using Chlorophyta as the out group, construct a cladogram to show the evolutionary relationship of the following phyla: Chlorophyta, Bryophyta, Anthophyta, Coniferophyta, andpterophyta.

  -protein x is a protein that is destined for secretion at

-protein x is a protein that is destined for secretion at the plasma membrane of one of our cells. show the cellular

  Describe the anatomy and histology of the cornea

Describe the anatomy and histology of the cornea, what is the function of each layer of the cornea and why is the cornea transparent?

  Regulation of physiological processes experiment

How does the concentration of a hormone affect the physiological response and the presence of one hormone block alter the action ofanother? Does the concentration of sugaravailable to the embryo alter the hormone response andthe embryo it self have..

  Evaluate and contrast the philosophies

Evaluate and contrast the philosophies of John Locke and Charles Darwin as they relate to developmental theory. In designing the drug that would bind to the active site and jam it, researchers must use which type of molecule.

  Describe the deficiency relationship of trace minerals

Describe the deficiency/toxicity relationship of trace minerals and why other nutrients do not have similar toxicity conditions.

  Evolutionary mechanism leads to adaptation

Which evolutionary mechanism leads to adaptation? Which can lead to loss or fixation of alleles particularly in small populations? How is fixation defined?

Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd