Define the vmax and km of the enzyme catalyzed reaction

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Reference no: EM13647028

Estimate the Vmax and Km of the enzyme catalyzed reaction from both of the plots. Compare your results from the two methods and explain why they are slightly different.

[S] (µmol/L) Vo (mM/min)

0.05 0.22
0.1 0.34
0.2 0.53
0.4 0.74
0.8 0.91
1.6 1.04
3.2 1.16

[S] vs Vo

Vmax = 2.138

Km=-4.31

Lineweaver-Burk plot

Vmax=1.1326

Km= -5.316

Reference no: EM13647028

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