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Which of the below describes the correct order in which procedures are carried out when using the Southern Blot technique?
(a) Cutting the DNA with restriction enzyme, blotting the DNA, electrophoresis of DNA, hybridizing the DNA with probe.(b) Cutting DNA with restriction enzyme, electrophoresis of DNA, blotting the DNA, hybridizing the DNA with probe.(c) Blotting the DNA, electrophoresis of DNA, hybridizing the DNA with probe, cutting DNA with restriction enzyme.(d) Electrophoresis of DNA, hybridizing the DNA with probe, cutting DNA with restriction enzyme, blotting the DNA.(e) Cutting DNA with restriction enzyme, hybridizing the DNA with probe, electrophoresis of DNA, blotting the DNA.
calculate as many points on the curve as needed to discern its basic charcteristics.how to plot points on a graph
What is the main difference between enveloped and nonenveloped viruses? How does this affect entry of each kind of virus into the host cells? Variances are expressed.
On physical assessment, nurse determines that his ankles and feet are very swollen. Which of these symptoms reflect left-sided heart failure and which reflect right-sided heart failure, and why give appropriate reason.
Do you really think the age of the man is main in determining the chance of chromosomal abnormalities? Why or why not with appropriate reasons. Describe a single meiosis event.
though , the addition of a trace of uniformly labeled [14C]acetyl-CoA in the presence of an excess of unlabeled malonyl-CoA to a soluble liver fraction yields palmitate labeled with 14C only in C-15 and C-16.
The phosphorylation of ADP to form ATP stores approximately 7.3 kcal per mole o ATP. What is the approximate efficiency of cellular respiration for a "mutant" organism that produces only 29 mole of ATP for every mole of glucose oxidized, rather than ..
When the differential is obtained what is the meaning of the percentage of.define What are the clinical scenarios.
Complex, Simple and Hypercomplex (Endstop cells) cells. Layer 4C and synapse onto simple cells. Whether or not they aer bars of light Simple cell is listening to a group of LGN cells. So each increment of 2.5 degrees has different simple cells.
The brown and scarlet mutations assort independently. If fruit flies that are heterozygous for both of these mutations are intercrossed, what sorts of progeny will they create, and in what proportions? Describe how you got the answer.
After contact with a patient spinal fluid a lab technician develop fever nausea and purple lessions on her neck and extremities. A throat culture grew gram negative diplococci. What is genus of the bacteria.
What is most likely genotype of the father? Mother? If the seventh child had blue eyes what could you conclude about parents genotyoes. What possible dissimilarity between these forms would give chemists a clue that open-chain form was not present.
Bisphenol A (BPA) binds to estrogen receptor with a Kd of 2.1 x 10-7 M and an association rate of 1.3 x 106 M-1s-1. Calculate the dissociation rate, kd, including appropriate units.
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