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Scientists are working on purifying PFK-1C from rabbit brain tissue. The rabbit brain tissue was homogenized and centrifuged to remove insoluble material. Next, the soluble preparation was loaded on top of an ATP-Sepharose column. This is an affinity column in which ATP is covalently linked to a polysaccharide bead. This sample is loaded on top of the column, washed with a low-salt buffer, followed by a wash with a high salt buffer. What is the rationale for this procedure and what would the expected elution profile look like? (Keep in mind PFK-1A Mr = 84000 dal, PFK-1B Mr = 80000dal, PFK-1C Mr=86000 dal)
Alcohols can be oxidized by chromic acid derivatives. One such reagent is pyridinium chlorochromate, (C5H5NH )(ClCrO3-), commonly known as PCC
Please show your work, and I will rate you! TWO PART QUESTION A)What is the rate constant of a first-order reaction that takes 277seconds for the reactant concentration to drop to half of its initial value
how many moles of sodium hypobromite should be added to 1.00 of 5.0times10-2 hypobromous acid to form a buffer solution
A 10mL aliquot of this solution, diluted to 25mL, yielded a signal of .235 units. A second 10 mL aliquot was spiked with 10mL of 1ppm standard solution of the analyze and diluted to 25mL. The signal for the spiked sample was .502 units. What is th..
mixing solutions of k2so4aq and bacl2aq produces an insoluble salt. which is the correct list of the ions which will
a man has sickle cell anemia, and an examination of his blood shows that a point mutation has caused the
producing a mixture of CO and CO2. The total pressure is 0.640 atm. What is the partial pressure of CO?
Assume that 0.406 mol Carbon (g) is placed in a cylinder that is expanding. The barometric pressure on the outside of the cylinder is constant at 1.00 atm and the temperature is 300.0 K.
a chemical reaction release 1240 J of energy. the energy is all absorbed by water (423 g) containing the reactants. if water was initially at 19.6 degrees, what will be it final temperature
How many resonance peaks should be observed in the hydrogen - decoupled C 13 - NMR spectrum of each of the following compounds?
Write as a superposition of 3 eigenstates, indicating the numerical value of each coefficient. Using the coefficients, demonstrate that is normalized.
Choose one of the oxidative protection enzymes, other than superoxide dismutase. Describe the metal containing active site, the general structure of the protein and the specific chemistry it catalyzes.
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