Define the physical interactions of the molecules

Reference no: EM13165077

1. If bovine LDH is known to have a temperature optimum around 37°C, why is it important to purify it at 4°C?

2. Explain why you should not lose any LDH activity when you centrifuge a crude sample at 20,0000 x g.

3. Explain why two different percent saturation levels of ammonium sulfate are used in these experiments.

4. Explain the physical interactions of the molecules that lead to proteins falling out of solution in high salt. What is the driving force of protein precipitation?

Reference no: EM13165077

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