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1. You have a 2 kb plasmid, and you want to insert a gene thatgives ampicillin resistance that is 500 bp in size. You also wantto insert a gene for penicillin resistance that is 435 bp in sizeand your PCR product, which is 250 bp in size. What would the totalsize of the plasmid be following the insertions?
2. Why would a different column, one unlike the silica gel purification column used in the protocol, cause anunsuccessful transformation?
3. What would happen if you forgot to heat shock the E. coli cells during the transformation reaction?
Is kinesin or dynein used for the following processes?
Is high fructose corn syrup bad for you? Explain your answer. To answer this question, you must do some research to find out what corn syrup is and what research has been done. Two credible sources are needed.
Results of this analysis show that one of the most rapidly reassociating classes of DNA is substantially reduced in the older individual with respect to the 2 year-old. How can you describe this finding.
A certain cell, which is surrounded of an extracellular, hypertonic environment, needs to move a molecule of sucrose out of the cell. What type of transport mechanism would it use?
Explain the role of passive diffusion in drug uptaking process, and how when designing a drug, you might increase its ability to be taken up by cells.
Create and label a generalized life cycle of Fungi with special reference to ploidy. Label plasmogamy and karyogamy in the life cycle.
What were some of the effects of the naturalistic world view upon science? Upon society at large?
Describe the substrates/products that link the light reactions of photosynthesis to the Calvin cycle.
You are becoming aware of the diversity and abundance of bacteria, and that we always have some uncertainty about the identity of an isolate. Describe the Hershey Chase experiment for determining the hereditary information in a virus?
In whippets, wild-type dogs are skinny. A mutation in the myostatin gene causes dogs to become more muscular. Dogs containing one copy of the mutant myostatin gene are medium-muscled; dogs in which both copies of the myostatin gene are mutant are ..
Describe what would be the specific probable effects on the cell if each of these organelles were lost. Try to avoid picking the same pair as other students in your class.
The structure of a mammalian hormone oxtocin, a nine-amino acid polypeptide as follows: cys-tur-ile-glan-asn-cys-pro-leu-gly. At the carboxyl end, the -OH is replaced by an amino group.
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