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Sequencing several genomes, including that of the zebra fish, has revealed a number of receptors and transporters. In one case, positional cloning of the zebra fish golden gene identified a putative cation exchange protein called SLC24A5. When the golden gene is mutated, the normally black horizontal stripes are pale or golden in appearance because the amount of black pigment or melanin is greatly reduced. Design an experiment to identify where the protein encoded by the golden gene is expressed and localized in the zebra fish. What is the term used when a mutant gene and its phenotype, for instance golden in zebra fish, is rescued by expressing an orthologous gene from another animal?
a woman produces an egg where the X chromosme underwent a nondisjunction event in meiosis 1. if this egg is fertilized by a normal sperm with an X chromosome, what would the sex of the offspring be?
Determine which of the following relationships corresponds to the lcoation of the isthmus of the thyroid gland?
If a cell were treated with an inhibitor that would cause microtubules to fall apart, which of the following would NOT be affected?
Write out the reactions of the glycolytic pathway starting from glucose to pyruvate. Starting with radiolabeled glucose labeled in the C1 position, where does the label ends up in pyruvate? (e.g. use a highlighter pen or an asterisk)
It has been shown that the repair of UV induced dimers in a gene is coupled to transcription. Suggest a model for this and any experiments you would use to test your model.
Which of the following is NOT a cis-acting sequence.
A young researcher at National Institute Health and interested in eye development, and hoping to find a cure to blindness in your lifetime
Show the restriction enzyme cut sites and the base pair distances between the cut sites on a circle. Bam HI= 190bp, 380 bp. Hind III= 570 bp. EcoRI= 165 bp, 405 bp. BamHI and HindIII= 30 bp, 190 bp, 350 bp. BamHI and EcoRI= 75 bp, 90 bp, 115 bp, 2..
What roles does natural variation play in the process of natural selection and, ultimately, evolution?
What happens to transcription at the lac operon when lactose is absent and present explain your answer.
You have 0.05 mL of an undiluted culture at a concentration of 3.6 x 10^6 CFU/mL. You then add 4.95 mL sterile diluent. What is the dilution, and what is the final concentration of cells?
The matrix used in experiment has a fractionation range 20000 to 5x10^6 Daltons. Would you see this matrix purify a plasmid that had a molecular weight.
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