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A kan-r, tet-r plasmid is treated with the restriction enzymeBglI, which cleaves the kan (kanamycin) gene. The DNA is annealed and ligated to a BglI digest of Neurospora DNA and thenused to transform E. coli.
a. What anitbiotic would you put in the growth medium to ensure that each colony has the plasmid?
b. What antibiotic-resistance phenotypes will be foundamong the resulting colonies?
c. Which phenotype will contain Neurospora DNA inserts?
If you were to carry out temperature experiments at a higher or lower [S], what effect would [S] have on the temperature optimum for amylase? Explain.
Opportunistic pathogens can be a big problem after someone gets treated with antibiotics. Is an opportuistic infection of the gut.
The red kernel color in wheat is caused by the presence of at least one dominant allele from each of the two idependently segregating gene pairs.
Which of the following is easiest to alter using dna technology?
Although respiration occurs in all living cells, different cells respire at different rates. Explain why. How could you predict the relative respiratory rates of different tissues in fish by microscopic examination of cells?
Which of the following is TRUE about enzymes?
What is the difference between decontamination, sterilization, sanitization, and degermation? Which one offers the highest level of control? Which one offers the lowest level of control?
In patients with chronic myelogenous leukemia, an odd chromosome is seen in all the cancerous cells. A small piece of chromosome 9 is connected to chromosome 22. This 9:22 translocation is called the Philadelphia chromosome. How could a translocat..
An isolated field of corn was found to be segregating for yellowand white endosperm. Yellow is governed by a dominant allele and white by its recessive allele
A newborn has a rare autosomal recessive disorder. It occurs with a frequency of 1/70,000. There is no history of this disorder in either the father or mother's family. What are the chances that two heterozygotes will meet and have an affected chi..
You have a constitutive mutant, but you are not sure if it is constitutive due to a repressor mutation (i-) or an operator mutation (o^c). What would be a good experiment to determine whether the phenotype is due to a defective repressor (i-) or t..
Determine which of the following methods can be used to identify the mesodermal cells that give rise to the vertebrate limb?
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