Reference no: EM132785717

1. Compare and contrast biotechnology, genetic modification, and recombinant DNA technology.

2. Identify the roles of a clone and a vector in making recombinant DNA.

3. Compare selection and mutation.

4. Define restriction enzymes, and outline how they are used to make rDNA.

5. List the four properties of vectors.

6. Describe the use of plasmid and viral vectors.

7. Outline the steps in PCR, and provide an example of its use.

8. Describe five ways of getting DNA into a cell.

9. Describe how a genomic library is made.

10. Differentiate cDNA from synthetic DNA.

11. Explain how each of the following is used to locate a clone: antibiotic-resistance genes, DNA probes, gene products.

12. List one advantage of modifying each of the following: Escherichia. coli, Saccharomyces cerevisiae, mammalian cells, plant cells.

13. List at least five applications of DN technology.

14. Define RNAi.

15. Discuss the value of genome projects.

16. Define the following terms: random shotgun sequencing, bioinformatics, proteomics.

17. Diagram the Southern blotting procedure, and provide an example of its use.

18. Diagram DNA fingerprinting, and provide an example of its use.

19. Outline genetic engineering with Agrobacterium.

20. List the advantages of, and problems associated with the use of genetic modification techniques