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You have isolated a unique mammalian protein, alpha, that recruits other partners in its normal function in the cell. Using a yeast two-hybrid system you have identified a potential binding partner, beta, for your bait protein.
Assuming that you could express and purify both components, describe TWO different experimental methodologies (including SALIENT details) that you would conduct to determine whether the two proteins interact in vivo in a mammalian cell line?
If you a litre (1000mL) bottle of oil in your engine, how much heat is produced in kcal?In BTU To solve this problem you must know the density of motor oil is .82 g/mL.
The sudden opening of the epiglottis and vocal cords releases this air explosively. Just prior to this release, what is the approximate pressure of the gas inside the lungs?
In the acid-catalyzed dehydration of alcohols, an alkene and water are the reactions products. What method of separation has been used in order to collect samples
Why is it an important concept that atoms generally appear in whole number molar ratios to each other in chemical compounds?
Determine the maximum amount of P4S10(444.545 g/mol) that can be produced from 18.27 grams of Phosphorous (123.895 g/mol) and 9.42 g of sulphur (256.52 g/mol)
Calculate the change in its standard molar Gibbs free energy when benzene is heated from 25 ºC to 45 ºC.
What volume will carbon dioxide originally 30.0 mL at 27°C have at 77°C
If iron is oxidized to Fe2+ by a copper(II) sulfate solution, and 0.704 grams of iron and 12.6 mL of 0.581M copper(II) sulfate react to form as much product as possible,
what is the value of equilibrium constant Kp if the total pressure is 11 atm?
collected over water at 22 degrees C and the total pressure of the wet gas were 710 mm Hg, what would be the partial pressure of the oxygen?
The reaction, seen below, is carried out in a constant-volume flask. Initially only dimethyl ether is present and the pressure is 0.350 atm. What is the pressure of the system after 8.0 minutes? Assume ideal behavior.
Calculate the number of grams of alcohol present in 1.00 L of 78 proof gin. The density of ethanol is 0.798 g/mL.
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