Define enzyme molecules to have substrate bound

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Reference no: EM13160824

Initially you measure values of Vmax=4.5 uM/min and Km=25 uM for a reaction. a) Suppose you want to set up a reaction with twice the enzyme concentration and a substrate concentration that will yield a reaction velocity of 4 uM/min. What would the appropriate substrate concentration be? b) At what substrate concentration would you expect 30% of the enzyme molecules to have substrate bound?

Reference no: EM13160824

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