Reference no: EM132439759

Problem:

Applying the Michaelis-Menten Equation IV An enzyme is found that catalyzes the reaction X ? Y. Researchers find that the K_m for the substrate X is 4 μM, and the k_cat is 20 min^-1.

(a) In an experiment, [X] = 6 mM, and V₀ = 480 nM min^-1. What was the [E_t] used in the experiment?

(b) In another experiment, [E_t] = 0.5 μM, and the measured V₀ = 5 μM min^-1. What was the [X] used in the experiment?

(c) The compound Z is found to be a very strong competitive inhibitor of the enzyme, with an α of 10. In an experiment with the same [E_t] as in (a), but a different [X], an amount of Z is added that reduces evolved to achieve catalytic perfection? Explain your answer briefly, using the kinetic parameter(s) that define catalytic perfection.. What is the [X] in this experiment?

This dealing with biochemistry and I need help on this question. Thank you and have a great day.