Define capabilities for single-cell analysis

Assignment Help Management Theories

Reference no: EM131445157

Question: To scale down biochemical reactions and provide capabilities for single-cell analysis, a German company named Advalytix (Munich) has developed an on-chip PCR platform involving a microscope slide containing 48 hydrophilic spots (although earlier versions had 60 spots). Each ‘ anchor ' spot on this AmpliGrid holds 1 μ L of solution containing the reaction components necessary for PCR. Because of the chemical nature of the hydrophilic spots, liquid is attracted to the defined positions and forms a small bead, which can then be covered with 5 μ L of mineral oil before thermal cycling is performed in order to prevent evaporation. The entire chip is then placed in a thermal ng steps. Full DNA profiles down to 32 pg of DNA template have been achieved with a commercial STR typing kit. Although it is challenging to pipet the small volumes necessary to perform this work, this technique is cost effective due to reduced reagent consumption.

Reference no: EM131445157

Questions Cloud

Value forlead bromide : ThePb2+concentration in a saturated solution oflead bromideis measured and found to be1.18×10-2M. Use this information to calculate a Ksp value forlead bromide.

Equilibrium concentrations of reactants and product : Calculate the equilibrium concentrations of reactants and product when0.229moles ofH2and0.229moles ofI2are introduced into a 1.00 L vessel at698K.

Capacity of the phosphate buffer : Calculate the expected buffer capacity of the phosphate buffer based on the number of moles of HPO4 2- (Hint: You know the concentration and the volume). In other words, how many moles are needed to break the buffer?

How the writing process can help you advance your education : Provide your thesis statement and begin creating the informal outline for your Continuing Academic Success essay assignment. Include the following in your outline this week:The benefit of creating educational goals, including at least one education..

Define capabilities for single-cell analysis : To scale down biochemical reactions and provide capabilities for single-cell analysis, a German company named Advalytix (Munich) has developed an on-chip PCR platform involving a microscope slide containing 48 hydrophilic spots.

Write python code to implement a basic number guessing game : CSIS1101- Write Python code to implement a basic number guessing game. The program will choose a random number between 1 and 9 (inclusive) using randint() .

What strategies on the writing process could you continue : focus on the writing process and the thesis statement, need about 100 words answereing the question: What strategies on the writing process could you continue to build on as you continue your studies?

Discuss about the racial problems in the us : Michelle Alexander says, "So many aspects of the old Jim Crow are suddenly legal again once you've been branded a felon. And so it seems that in America, we haven't so much ended racial caste, but simply redesigned it." What does she mean by this?

What does just-in-time inventory management : What does Just-in-time inventory management have to do with the Carle Heart Center in Urbana, Illinois? The Carle Heart Center is one of the most sophisticated cardiac care facilities in the Midwest.

User Account

All Pages