Define a standard spectrophotomer is linear to an absorbance

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Reference no: EM13558462

A Technologist is performing a colorimetric assay with a ELISA absorbance reader. The assay's color, when tested in a standard spectrophotomer is linear to an absorbance of 4.0, however the ELISA reader has a range of linearity which only extends to an absorbance of 1.2. Several of the wells has an approximate absorbance of 1.5, and are diluted 1:1 with a buffer. The absorbance values remain the same.

Why didn't the absorbance values change (considering Beer's Law)

Reference no: EM13558462

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