Deficient in producing tryptophan

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You have an E. coli auxotrophic mutant that is deficient in producing tryptophan, an essential amino acid required for its growth. This mutant is also F- and the deficiency is as a result of a mutation in a core gene in the tryptophan synthesis pathway.

Devise and explain an experiment to show how you can use conjugation with a suitable donor strain to quickly restore functionality of the tryptophan pathway

Reference no: EM132556214

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