Reference no: EM133378279

Cellular Basis of Disease

Just need t test graph plotted and discussion

Assessment task: You need to report, analyse and present your research findings in a laboratory report.

A patient is presenting with lower back pain, haematuria and loss of appetite. As such a clinician believes the patient may have kidney disease/cancer. You have been asked to investigate in the lab.

In laboratory sessions you will perform H&E stains, immunophenotying assays and ELISA.
• H&E will provide a picture of the microanatomy of a healthy kidney vs a diseased kidney.
• Immunophenotyping will provide the basis of disease diagnosis.
• Sandwich ELISA will measure IL-6 production from the serum of a patient with a healthy kidney vs a diseased kidney.

This report will need to include an introduction, justification of methods, results and discussion. Word counts for each section are suggestions only.

Introduction (450 words) - should contain a critical review of the literature surrounding renal cell carcinomas and more specifically clear cell renal cell carcinoma (correct reference citation using APA formatting).
• Review of clear cell renal cell carcinoma pathophysiology
• Epithelial and mesenchymal transition, should integrate cell-cell adhesion within the development of clear cell renal cell carcinoma.
• Case study introduced
• Aims included

Justification of methods (350 words) - You will undertake 4 lab sessions these are histology, immunophenotyping and ELISA.
• Explain the research design of the study, think about outcomes of the experiments.
• Briefly integrate in the underlying theory.

Results (450 words) -Descriptive text with integrated Figures/Table. The results should be presented in a logical order and Figures/Tables should be clearly presented and labelled. Data will be collected during the mandatory laboratory sessions.
• For each experiment/figure: (a) briefly introduce what you did and why; (b) then show the Figure/Table; (c) then clearly describe the Figure. The data that should be presented is H&E staining of healthy vs control, immunophenotyping data healthy vs control and your ELISA data.
• Do not include any raw data or calculations, you may need to refer to this in text but should not feature in the results section.

Discussion (750 words) - You should critically review your data and explain your results using molecular pathways. This section should be driven by the literature.
• Initially (briefly) review main aims and integrate in main findings.
• Explain what your results show but more importantly link to literature to explain your results i.e.how does IL-6 link to patient prognosis?
• Your results indicate cancer cells are signalling to "normal" kidney cells, what is the importance of this.
• You should explain importance of your result for the patient, i.e. possible diagnosis and prognosis based on your research findings.