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Question: Ligation of the multiple ? fragments and pBLU produces a number of different hybrid molecules, although not as many as you might expect. Each clonable fragment must have one BamHI and one HindIII end, to combine with the BamHI and HindIII ends of the pBLU backbone and form a circular recombinant plasmid. Only 5 of the 13 BamHI/HindIII framgnets from the linear ? genome satisfy this requirement: 493 bp, 784 bp, 2,396 bp, 2,409 bp, and 4,148 bp. A sixth clonable fragment of 9,866 bp is derived from the COS circle. Multiple copies of a single fragment or combinations of two different ? fragments can also be ligated into a single pBLU backbone. Three fragment plasmids can be constructed by combining the pBLU backbone with one of hte five fragments having one BamHI end and one HindIII end and with one of the eight fragments having two BamHI or two HindIII ends. Constructs with more than one unit of pBLU are also formed. Oly constructs with at least one unit of pBLU backbone contain an origin necessary for replication and the ampicillin-resistance gene required for growth on selective media.
1. Make scale drawings of all potential two-fragment (vector + insert) recombinant plasmids. Include the total bp size, fragment bp sizes, and the locations of BamHI sites and HindIII sites, the origin, the amplicillin-resistance gene, and the lacZ gene.
Both the endocrine and nervous system are main regulating systems of the body though the nervous system has been compared to an airmail delivery system and the endocrine system to the pony express. Briefly explain the comparison.
Most of us can't even imagine a world without oxygen. Though, as you learned earlier, chemically oxygen is a powerful oxidizing compound. What effect(s) would the increase in oxygen levels of atmosphere have on the organisms that existed at that time..
Assume you are attempting to determine if the new DNA sample you have just received contains linear or circular molecules. Digestion using either RsaI or SauI alone produces two fragments.
explain how an action potential, originating at the axon hillock, is propagated along myelinated and nonmyelinated axons.How are graded potentials made
A hypothetical enzyme in which the active site is complementary to the substrate will.
Spatially explicit model shows no evidence of admixture between Eurasians and Neanderthals
Chimpanzees have 24-pairs of chromosomes. Chimpanzee somatic cells contain DNA double helices.
Explain three examples of wehre fossils have been used to recontruct behavior of extinct animals.
In the aerials competition in skiing, the competitors speed down a ramp that slopes sharply upward at the end. The sharp upward slope launches them into the air, where they perform acrobatic maneuvers.
Complete the Punnett squares for a cross between the male with blood type B and a female with blood type AB. Build two Punnett squares and answer the following given questions about them.
Question: A patient in your care has a total body water volume of 42 Liters, a hematocrit of 40 percent an extra celllular fluid volume of thirteen liters and a plasma osmolarity of 300 mOsm/ liter.
Suppose an analyzer created a purebred strain of these smart mice and started breeding them with other mice to map the gene responsible for the phenotype.
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