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EXERCISE - GENETIC MUTATIONS
1. Translate the following sequence: AUG GAG GUC UUU AAG AGA CAU UUA GAU GUA GCC CUU AGU GAU GUU UAG
2. Create one or more point mutations in this sequence: AUG GAG GUC UUU AAG AGA CAU UUA GAU GUA GCC CUU AGU GAU GUU UAG
3. Translate your mutated sequence.
4. Now, create a frame-shift mutation by adding one or more bases. AUG GAG GUC UUU AAG AGA CAU UUA GAU GUA GCC CUU AGU GAU GUU UAG
5. Translate your mutated sequence.
6. Create a frame-shift mutation by deleting one or more bases. AUG GAG GUC UUU AAG AGA CAU UUA GAU GUA GCC CUU AGU GAU GUU UAG
7. Translate your mutated sequence.
8. Align the amino acid sequences that you determined for each of the four transcripts.
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What is a mutant screen? What is it used for? How does a restriction enzyme “know” where to cut? How does the process of electrophoresis allow us to determine the size of DNA fragments? What is the most common method for visualizing DNA on a gel? How..
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