Consider a chromatogram containing a single analyte

Reference no: EM13171747

1. Consider a chromatogram containing a single analyte with retention time of 4 minutes. The mobile phase elution time is 0.2 minutes. What is the capacity factor (k') for the analyte in question?

2. If 50 ?L of 0.03 molar analyte (from question 1) is injected into the column, how many moles of analyte are in the stationary phase during the separation?

3. If the mobile phase flow rate is 0.4 m/min, what is the analyte linear migration rate, and how long is the column?

4. If a second analyte in the column has a retention time of 6.3 minutes, what is the capacity factor for this second analyte? What is the linear migration rate of the second analyte.

5. If the column is a packed bed containing C18 (nonpolar) stationary phase, which analyte (1 or 2) is more polar? Why?

Reference no: EM13171747

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