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Your third task is to mutate the SID-1 promotor region. Below is 120 bp of the SID-1 genomic sequence ending with the start codon for the SID-1 ORF. A putative -10 equivalent in this promoter is the TATA sequence. You're interested to know if the -10 consensus sequence for prokaryotes could increase the expression of this eukaryotic gene.
A) What seven base pair primer pair could we use to mutate the current promoter to look more like a consensus prokaryotic promoter? Write the sequence and 5', 3' ends of your primers below.
B) You also want to PCR up this whole 120 bp sequence. You are told to order 10 bp PCR primers that are designed to bind to each end of this sequence. i) You object to using 10 bp sequences for the PCR experiment. Explain your reasoning.
ii) You're assured that the 10bp length will not be a problem. Given that guarantee, do you think this primer pair could work? Explain your reasoning.
It is a very curcial concept to understand how the immune response is mounted against viruses, bacteria, protozoans and helminthes. For an effective immune response, both innate and adaptive immunity should work together.
This Project report elaborates a critical review of important elements attached to Advanced Glycated End Products (AGEs). It is very crucial to understand the process called Millard reaction.
Soil stabilization is the permanent physical and chemical alteration of soils to enhance their physical properties. Stabilization can increase the shear strength of a soil and control the shrink-swell properties.
This assignment has three parts which contains questions related to Microbiology. It contains basic principles of microscopy, staining techniques in microbiology and microbial growth in the food industry.
Lipid metabolites are often seen as key elements in cellular signaling. Is this unique? Please provide several examples of the function of lipids as key elements in signal arrays and list the biologic functions these signals affect?
Please describe how one might search for chemical structure, biologic function relationships, involving small molecular weight lipophylic compounds. Provide one example.
Write a case study which detailing a scenario of a patient being investigated in the Haematology laboratory.
The use of PCR and genetic approaches in biotechnology
Glucose oxidase is an enzyme that can be used for measurements of glucose levels by combining this reaction with an oxygen probe.
What phenotypic ratio would you get if you crossed a white mouse and a heterozygous brown mouse?
Prepare an essay on nosocomial infection.
To increase the awareness of monitoring and recording the blood pressure of patients and practice measuring blood pressure in a safe environment.
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