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A scientist wants to analyze the transcriptome of Escherichia coli. To do so, he grows E. coli in a flask, takes a sample from the culture, and extracts the nucleic acids from the sample. She then uses an enzyme called DNase to remove the DNA, leaving only RNA. The RNA is then converted to complementary DNA (cDNA) using a reverse transcriptase. This cDNA, which is more stable than the RNA from which it was synthesized, is broken into small fragments (< 400 bp), and these fragments are then sequenced. The resulting transcriptome dataset (RNASeq) contains millions of sequence fragments corresponding to the transcripts from the original sample. However, upon reviewing his lab notes, the researcher notices he did not add the recommended amount of DNase. He suspects that his dataset may contain sequences originating from DNA, in addition to those from RNA. Describe two patterns that he could look for in the data to confirm that the sequence data reflect RNA, rather than the DNA from which the RNA was transcribed. For each, describe why such a pattern would be expected for RNA, but not DNA. Note: the E. coli genome has been sequenced in its entirety, i.e., the sequences of all genes and other genetic elements are known, as is the location of these features on the chromosome.
It is a very curcial concept to understand how the immune response is mounted against viruses, bacteria, protozoans and helminthes. For an effective immune response, both innate and adaptive immunity should work together.
This Project report elaborates a critical review of important elements attached to Advanced Glycated End Products (AGEs). It is very crucial to understand the process called Millard reaction.
Soil stabilization is the permanent physical and chemical alteration of soils to enhance their physical properties. Stabilization can increase the shear strength of a soil and control the shrink-swell properties.
This assignment has three parts which contains questions related to Microbiology. It contains basic principles of microscopy, staining techniques in microbiology and microbial growth in the food industry.
Lipid metabolites are often seen as key elements in cellular signaling. Is this unique? Please provide several examples of the function of lipids as key elements in signal arrays and list the biologic functions these signals affect?
Please describe how one might search for chemical structure, biologic function relationships, involving small molecular weight lipophylic compounds. Provide one example.
Write a case study which detailing a scenario of a patient being investigated in the Haematology laboratory.
The use of PCR and genetic approaches in biotechnology
Glucose oxidase is an enzyme that can be used for measurements of glucose levels by combining this reaction with an oxygen probe.
What phenotypic ratio would you get if you crossed a white mouse and a heterozygous brown mouse?
Prepare an essay on nosocomial infection.
To increase the awareness of monitoring and recording the blood pressure of patients and practice measuring blood pressure in a safe environment.
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