User Account

All Pages

Confirm dna column for the remaining columns

Assignment Help Biology
Reference no: EM13893483

Based on the following information how do I solve for 10x buffer, uls HOH, ul Enzyme, and Total?

Setup a digest that contains 10 ug of DNA/Sample, 3.5 ug/ul stock DNA (from experiment with 5ul of stock diluted with 995 H2O), 40 units of HAE III enzyme, and enzyme stock of 12 units/ul.

Sample ul DNA uls 10xBuffer uls HOH uls Enzyme Total ul
1 10ug/3.5 ug/ul

Please confirm DNA column, and demonstrate or provide formulas for the remaining columns.

Reference no: EM13893483

Questions Cloud

Management studio for database design : 1.Use the Management Studio to create a new database called MyWebDB using the default settings. (Ifthe database already exists, use the Management Studio to delete it and then recreate it.) 2. Use the Management Studio to create the following tables ..
What is mean and the standard deviation of the accumulation : What is the mean and the standard deviation of the ACCUMULATION? What is the probability that the ACCUMULATION is over $200,000? over $240,000?
Describe the hypothetical scenario you have developed : Describe the hypothetical scenario you have developed. Explain the steps for setting up a good strategy-monitoring and adjustment program, as it relates to this scenario
Define competitive irrationality : Define competitive irrationality
Confirm dna column for the remaining columns : Based on the following information how do I solve for 10x buffer, uls HOH, ul Enzyme, and Total? Setup a digest that contains 10 ug of DNA/Sample, 3.5 ug/ul stock DNA (from experiment with 5ul of stock diluted with 995 H2O), 40 units of HAE III en..
Have you ever used a fitness tracking device : Survey Questions - How old are you? Have you ever used a fitness tracking device? How much physical activity do you have each day
What is the importance of statistical data analysis : What is the importance of statistical data analysis? What are the appropriate published literature in my area of research?
Discuss sc johnson''s business model as described : Discuss SC Johnson's business model as described
About the business''s approach toward ethics and social : about the business's approach toward ethics and social responsibility

Reviews

Write a Review

Biology Questions & Answers

  Science fiction yet present in science

Which trait(s) is/are lacking in science fiction yet present in science

  Are there sufficent water molecules to associate

For fusion of a vesicle with it's target membrane to occur. The membranes have to be brought to within 1.5 nm so the two bilayers can join. Assuming that the relevant portion of the two membranes at the fusion site are circular regions 1.5 nm in d..

  Diaphragm and external intercostal muscles contract

When the diaphragm and external intercostal muscles contract: The size of the pleural cavity increases Intrapleural pressure decreases.

  Which of the following is true of fungi

Which of the following is TRUE of fungi?

  Explain how impact the immune system and respiratory system

explain how it can impact the immune system and respiratory system

  In brief keith requires to begin eating well-balanced meals

keith is a college student. he is extremely busy studying and working part-time. keith sleeps in late and therefore

  Discuss the implications for monetary and fiscal policy of

while the budget factored in a decline in australias terms of trade the fall in global commodity prices over recent

  Explain the importance of membrane structure

Describe the structure and function of each of the eukaryotic organelles. Explain the importance of membrane structure and function in the organization of living processes within cells

  Rhosopseudomonas is an anaerobic photoautotroph

Rhosopseudomonas is an anaerobic photoautotroph. How would you sterilize a mattress used by a patient with bubonic plague.

  Explain classical transcription activators like gal4p

Suggest two ways Rap1p might function so that it is necessary for transcription of Rap1pdependent genes. Please use diagrams to show how it might work.

  Voltage potential across the mitochondrial membrane

The ph of mitochondria matrix is 8.2 while the ph of intermembrane is 7.2. Determine the voltage potential across the mitochondrial membrane which would prevent hydrogen ions from flowing into matrix?

  Damage when encountered during replication

Pyrimidine dimers cannot be copied by the normal action of DNA polymerases, so DNA replication would stop when the polymerase encounters one of these dimers. What mechanism have cells developed to deal with this damage when encountered during replica..

Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

Submit Assignment

Academics

Major Subjects

Majors

Get In Touch

whatsapp: +1-415-670-9521

Phone: +1-415-670-9521

Email: [email protected]

TERMS & POLICIES

HELP & SUPPORT

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd