Reference no: EM131344025
1. In order to prepare the bovine serum albumin (BSA) standard for this assay, it would be necessary to dissolve a sample of BSA in water or in a buffer, and allow the protein time to dissolve, filter or centrifuge the solution (to remove any un-dissolved materials), and finally determine the exact concentration of the protein. UV absorption is a good way to determine the exact concentration. The published extinction coefficient for bovine serum albumin is E2801% = 6.6. The concentration unit % solution) here are a little odd, and dates to the day before the structure and molecular weight of the protein were known. A 1% solution is:
Or put another way a 10 mg/mL solution will have an A280 = 6.6.
Let's say that 5 mL of a BSA solution is prepared, its A280 is read and A280 = 0.82
What is the concentration of the solution in mg/mL?
2. Show how you would dilute the BSA solution made in Problem 2 to make 5 mL of exactly 1.00 mg/mL BSA.
3. Gelatin is an irreversibly hydrolyzed form of collagen that is used in pharmaceutical, cosmetic, and culinary products. The principle amino acids in collagen are glycine, alanine and proline, with small amounts of phenylalanine, traces of tyrosine and no tryptophan. Will UV spectroscopy be a sensitive way to measure concentration of gelatin? Why or why not?
4. If an experimenter found that the Bradford method using dye binding was not suitable for measuring the target protein, and that UV absorption was not sensitive enough, name another method that might be used to measure the protein.
What is sociobiology
: What is sociobiology? What contributions has sociobiology made to the study of criminality. Be sure to explain by using a real-world example.
|
Identify the theory of effectiveness that you are employing
: In light of the legalization of marijuana gaining steam across the nation, the legality of mandatory minimum sentencing laws have again come into question by many. The federal government's mandate of a five year sentence attached to the drug in th..
|
What kind of research questions lead to qualitative approach
: What kinds of research questions lead to a qualitative approach? If you chose to conduct quantitative research in your Week 4 proposal, how might you reframe the research question in a way that lends itself to a qualitative approach
|
What torts has edward committed against john
: What torts has Edward committed against John and how are the elements of those torts met in this instance?
|
Concentration of the solution
: Or put another way a 10 mg/mL solution will have an A280 = 6.6. Let's say that 5 mL of a BSA solution is prepared, its A280 is read and A280 = 0.82 What is the concentration of the solution in mg/mL?
|
What is the strongest metal
: 1) What is the strongest metal? 2) The strongest alloy? 3) Which salt has the highest vaporization point? 4) Which covalent molecule has the highest vaporization point?
|
Dual court system of the united states
: Major historical developments of the U.S. courts - Dual court system of the United States and steps of the pretrial process - Six steps necessary for appeal and Five philosophical reasons for sentencing criminals
|
Determine the maximum weight w that can be supported
: The breaking strength of the cable FG that supports the portable camping stool is 400 lb. Determine the maximum weight W that can be supported. Neglect friction and the weights of the members.
|
Percentage of the atoms of carbon
: A) In a living organism, the decay of C-14 produces 15.3 disintegrations per minute per gram of carbon. The half-life of C-14 is 5730 years. A bone sample with 4.0 g of carbon has 17.5 disintegrations per minute. How old is the bone sample in year..
|