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Reconstitution, Volume of Water, and Ovalbumin
For your upcoming biochemistry experiment, a protocol indicates that you will need a 6.1 mM solution of the protein 'ovalbumin'. Looking around the laboratory, you find one vial of ovalbumin. The label and Certificate of Analysis that shipped with the ovalbumin provide the following information.
- Sigma catalog number A5503
- Albumin from chicken egg white (synonym: ovalbumin)
- >98% pure (agarose gel electrophoresis)
- Mol Wt: 44287 Da
- Form: Lyophilized powder
- Mass: 50 mg
Calculate the volume of water required to reconstitute (also referred to as solubilize) the entire contents of the vial of ovalbumin to a final concentration of 6.1 mM.
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