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The initial rates at various substrate concentrations for an enzyme-catalyzed reaction are as follows:
[S], M vo, M/min2.50 x 10-5 3.80 x 10-54.00 x 10-5 5.34 x 10-56.00 x 10-5 6.86 x 10-58.00 x 10-5 8.00 x 10-51.60 x 10-4 1.068 x 10-42.00 x 10-4 1.140 x 10-4
a) Does this reaction follow Michaelis-Menten kinetics?
b) Calculate the values of vmax and Km for the reaction.
c) How much of a noncompetitive inhibitor must be added to yield 90% inhibition? The degree of inhibition, i, is calculated by i% = (1-?) 100%, where ? = (vo)inhibition/ vo Assume KI = 2.9 x 10-4 M.
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Ethylene glycol, C2H6O2, is widely used as an automotive antifreeze. What is the minimum weight, in grams, of this compound that must be added to 10 kg of water to produce a solution that will protect an automobile cooling system from freezing at ..
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