Compute the values of vmax and km for the reaction

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Reference no: EM13227705

The initial rates at various substrate concentrations for an enzyme-catalyzed reaction are as follows:

[S], M vo, M/min
2.50 x 10-5 3.80 x 10-5
4.00 x 10-5 5.34 x 10-5
6.00 x 10-5 6.86 x 10-5
8.00 x 10-5 8.00 x 10-5
1.60 x 10-4 1.068 x 10-4
2.00 x 10-4 1.140 x 10-4

a) Does this reaction follow Michaelis-Menten kinetics?

b) Calculate the values of vmax and Km for the reaction.

c) How much of a noncompetitive inhibitor must be added to yield 90% inhibition? The degree of inhibition, i, is calculated by i% = (1-?) 100%, where ? = (vo)inhibition/ vo Assume KI = 2.9 x 10-4 M.

Reference no: EM13227705

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