Compute the theoretical ph of one of the buffer

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Reference no: EM13178897

In my Biochemistry lab this week we made buffer solutions. I had TRIS. I made 100ml of .1M solutions. The original ph was 9.15. I added HCl until ph matched the listed pka of 8.3. weight of TRIS is 1.235g.

(a) what is the ratio of A- / HA in your buffer are present in the solution?

(b) How many micromoles of A- and HA are present in the solution?

Calculate the theoretical ph of one of your buffer at 0^0 C. Assume that room temperature is 22^0 C. If none of your buffer is listed on the table of changing pka with temperature, do this problem for TRIS at ph 8.0.

Reference no: EM13178897

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