Compute the number of theoretical plates n

Reference no: EM13205233

Two isotopic compounds are produced in the synthesis of a new therapeutic drug which must be separated by repeated chromatography cycles. In each cycle, the solution containing both compounds must pass through a chromatography column with a length L = 75.0 cm. The column has N theoretical plates and the unadjusted relative retention is ? = 1.008.

A) Calculate the number of theoretical plates N in the column of length L in 20 cycles. The observed resolution is 1.81.

B) Now, calculate the plate height in the column in ?m.

C) Estimate the resolution in 4 cycles.

Reference no: EM13205233

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