Compute the mass of protein in the unknown

Assignment Help Chemistry
Reference no: EM13246302

In a protein determination, the color of dye changes to blue when it binds to protein, and the absorbance of light is measured. The data below were taken to calibrate the instrument. An unknown protein sample gave an absorbance of 0.867 ( corresponding to the average of three replicate analyses of the unknown sample). Calculate the mass (micrograms) of protein in the unknown. Calculate the absolute uncertainty for the answer.

Data:
Protein (micrograms): 0.00, 9.37, 18.73, 28.08, 37.44
Absorbance: 0.490, 0.720, 0.850, 1.110, 1.250

Reference no: EM13246302

Questions Cloud

Explain equations as a strong and weak : classify each solute represented in the following equations as a strong, weak, or nonelectrolyte: a)CH3-OH(l) H2O> CH3-OH(aq). b)MgCl2(s) H2O> Mg2+(aq) + 2Cl-(aq). c) HClO(aq) H+(aq) + ClO-(aq)
What is the mass of the ice that melts : 50-g of hot water at 65 degree C is poured into a cavity in a very large block of ice at 0 degrees C, What is the mass of the ice that melts
How did turkey end up a secular democracy : How did Turkey end up a secular democracy? It seems like so many countries in that area have ended up being dictatorships.
Determine the temperature increase of the ball in degree : A 15-kg iron ball is dropped onto a pavement from a height of 80m. Suppose half of the heat generated goes into warming the ball. What is the temperature increase of the ball in degree C
Compute the mass of protein in the unknown : An unknown protein sample gave an absorbance of 0.867 ( corresponding to the average of three replicate analyses of the unknown sample). Calculate the mass (micrograms) of protein in the unknown. Calculate the absolute uncertainty for the answer.
Treated in different regions and economic settings : What factors account for how African slaves were treated in different regions and / or economic settings in Latin America?
Compute the total concentration of lead dissolved solution : Calculate the total concentration of lead dissolved ([Pb]total) in a solution saturated with PbI2(s) and containing dissolved I- with a concentration of [I-] = 0.294 M. The solubility of lead is governed by the reactions shown below.
How fast is it going when it comes back down to earth : A rocket is fired from the ground but at 30m it runs out of fuel, How fast is it going when it comes back down to earth
How much empty space would be left in each structure : Which has a greater packing fraction, a diamond crystal structure or a copper crystal structure, how much empty space would be left in each structure

Reviews

Write a Review

Chemistry Questions & Answers

  Steps in the mechanism for the following reaction

Show all the steps in the mechanism for the following reaction, When benzene is mixed with deuterated sulfuric acid, deuterium is slowly incorporated onto the ring. Show the mechanism for this reaction and explain how this relates the sulfonation of ..

  Prior to placing piece of metal into the graduated cylinder

This assignment inhibits chemistry Laboratory Questions.

  Write the structures of the saytzeff elimination

Write the structures of the saytzeff elimination

  Calculate ph - chemistry questions

Chemistry Questions on Calculate P H

  How many mols of hydrogen can produce

how many mols of H 2 can produce

  Analysis of corrosion mechanisms

Analysis of corrosion mechanisms and preventative measures

  Chemical and pharmaceutical science

Write an equation for the formation of an acetal from reaction of excess methanol with benzaldehyde in the presence of an acid catalyst.

  Calculate the approximate sulphur

Calculate the approximate SO 2 mass emission in lb/day.

  What is the structure - stereochemistry

What is the structure (including functional groups)? Stereochemistry (racemic or single enantiomer)?

  Design a qualitative analysis scheme

Design a qualitative analysis scheme

  What will be the resultant pressure

What will be the resultant pressure when the stopcock is opened?

  The 1h nmr spectrum

Integrals for some of the resonances in the 1H NMR spectrum are higher than they should be due to the shear number of hydrogens in this compound

Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd