Compute the concentraion of the deprotoned form

Reference no: EM13184259

Data from a lab experiment to calculate the concentraion of the deprotoned form of p-nitrophenol in the solution that we made up which consisted of:

0.5 mL of 1.0 mM p-nitrophenol

9.5 glycine buffer

pH of buffer solution 9

pka of p-nitrophenol 7.15

Understand that the Henderson-Hasselback equation, but I'm not sure if I am setting it up right.

9=7.15 + log (x/0.5 mM)

x=3.54mM

To find 0.5 mM I just did M1V1=M2V2

Reference no: EM13184259

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