Compute the activity of the enzyme at vmax

Reference no: EM13192864

A student performed similar experiments as described in this lab exercise, but 200uL of enzyme was used per assay. This student found the Vmax of alkaline phosphatase to be .073 umole substrate hydrolyzed per minute. The concentration of alkaline phosphate in the stock solution used in the experiment was .25mg protein/mL. Calculate the activity of the enzyme at Vmax as umole substrate hydrolyzed per minute per mg of enzyme protein.

Reference no: EM13192864

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