Compound p binds with x to form complex px create the

Reference no: EM13469326

Compound P binds with X to form the complex PX. A series of solutions was prepared with the total P concentration remaining fixed at 1.00 x10-5M in all solutions. Both P and X do not absorb at 437 nm, but PX has an absorption maximum at 437 nm. The following table shows how the absorbance at 437 nm in a 5.00 cm cell depends of [X], using the assumption that [X]is approximately qual to XTbecause XT>>*P+.

[X] in M	A
No X added	0.000
0.00200	0.125
0.00400	0.213
0.00600	0.286
0.00800	0.342
0.0100	0.406
0.0200	0.535
0.0400	0.631
0.0600	0.700
0.0800	0.708
0.1000	0.765

Make a Scatchard plot of ΔA/*X+ versus ΔA in order to find the equilibrium constant K for this binding event.

Reference no: EM13469326

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