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Complete the following purification table

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Reference no: EM131129260

1. Complete the following purification table. Which step gives the best purification?

2. Define and/or explain the significance of All of the following.

a. Explain why a fluorescent amino acid, whether naturally occurring (tryptophane) or artificial (Aladan), might be of advantage in Biochemistry.

Step

Total VoIn me

Total Protein (mg)

Total Activity (units)

Specific
Activity
(units/mg)

Yield (%)

Purification
(n-fold)

Crude Cell extract

2,800

70,000

2,700

 

 

 

Ammonium Sulfate

3,000

25,400

2,300

 

 

 

Heat Treatment

3,000

16,500

1,930

 

 

 

DEAE Chromatography

80

390

1,630

 

 

CM Cellulose Chromatography

50

47

1,350

 

 

 

Bio-Gel A Chromatography

7

35

1,120

 

 

 

 

Reference no: EM131129260

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