Reference no: EM132761909

Answer by true or false. For each false statement, make correction one

A) Knock-out model is an animal with a de-activated target gene, which can be used to study the functions of the target gene. Knock-in model is an animal with a specific alteration in a target gene. To study a single pathological mutation (e.g. delta F508 of cystic fibrosis), a knock-in model is likely used.

B) Animal cloning technology is typically used to create transgenic animals with altered genes.

C) Green fluorescence protein (GFP) can be used as in vivo marker to monitor gene expression only because it is fluorescent.

D) Based on central dogma of biology, both transcription with RNA polymerase and translation within ribosome are required for a cell to express an active gene protein.

E) Either injecting the DNA construct into pronucleus of fertilized eggs or injecting the DNA construct into embryonic stem cells can be used to induce the DNA construct into cells for the homologous recombination process.

F) Both enzyme linked cellular surface receptors and G-protein coupled surface receptors are membrane proteins and consist of a single polypeptide chain. Whereas an enzyme-linked receptor has seven transmembrane segments, a G-protein-linked receptor usually has only one single pass.

G) Base on the Equivalent Circuit Model, when the drive force (I, the current of ions) is positive, the flow of the ions will be inward.

H) Action potential of excitable cells can propagate in dual directions.

I) Genomics is used to describe the study of an organism's entire genome, i.e. genes of a cell or tissue at DNA and RNA levels; and proteomics is the large-scale study of proteins coded by entire genome, in term of their structures and post-translational modifications and their associated functions.

J) Comparing to PCR amplification of DNA, DNA sequence analysis requires 2 primers and a mixture of dNTPs and ddNTPs.