Reference no: EM132520815

Molecular II. Comparative Proteomics Protein Electrophoresis and SDS - PAGE.

1. Briefly define proteomics and explain why proteins of higher eukaryotes are of so much interest.

2. Explain in simple clear terms what electrophoresis is.

3. Why is it important to use SDS buffer and heat to prepare the protein sample?

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1. In the electrophoresis set up, buffer was placed in both the center and the lower chamber. Why was that buffer placement essential?

2. Your SDS-PAGE gel ran for around 35 minutes. What would your results have been if you had run it for only 15 minutes? What would have happened if you had run the gel for three hours?

4. What reasons might there be for a discrepancy between the molecular data and the taxonomic classification?

5. Evolutionary geneticists would typically examine more than one kind of protein before concluding evolutionary relationships. Why does this practice make sense?

6. Peter Fimrite of the San Francisco Chronicle reported (February 2013), "Genetic testing of 1,215 fish take from 674 retail outlets, grocery stores and sushi bars throughout the United States between 2011 and 2012 found that 33 percent of the samples had been mislabeled, according to U.S. Food and Drug Administration guidelines... These fraudulent practices also carry potentially serious concerns for the health of consumers and for the health of our oceans and vulnerable fish populations." Can you see a use for SDS-PAGE that is not related to esoteric evolutionary studies?