Common in proteins that bind nucleotide-derived cofactors

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1) The protein has a GXXSXG motif, common in proteins that bind nucleotide-derived cofactors. Name three cofactors that could bind this motif.

2) Given the presence of this motif, you suspect your protein may be enzyme. What general type of chemistry do you surmise it to catalyze?

3) Your gene of interest is encoded in human cells. You wish to express recombinant protein in the bacterium Escherichia coli. A colleague in lab hears about your project and welcomes you to a stock of cDNA to aid in your cloning. Describe why this is necessary and what enzyme would you need if you had to generate your own cDNA?

Reference no: EM132439550

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