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Cloning a particular segment of dna

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You are interested in cloning a particular segment of DNA and wish to use a commercial cloning vector with a BamHI cloning site. However, your segment of DNA does not contain the appropriate restriction site. Discussing your problem with a college they suggest using PCR to address your problem. How can you use a PCR reaction to facilitate a successful cloning?

Reference no: EM132016872

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