Cloned dna sequence was used to probe a southern blot

Assignment Help Biology
Reference no: EM13527611

A cloned DNA sequence was used to probe a Southern blot. There were 2 DNA samples on the blot, one from white blood cells and the other from a liver biopsy of the same individual. Both samples had been digested with HpaII. The probe bound to a single 2.2 kb band in the white blood cell DNA, but bound to 2 bands (1.5 and 0.7 kb) in the liver DNA.

a ) What are possible explanations for this difference?

b ) How would it affect your answer if you knew that white blood cell and liver DNA from this individualboth showed the 2 band pattern when digested with MspI ?

Reference no: EM13527611

Questions Cloud

What are the most significant challenges facing : What are the most significant challenges facing organizations in today's dynamic market.
Fasciculus cuneatus-fasciculus gracilis-tectospinal tract : From the key choose the name of the tract that might be damaged when the following conditions are ovserved.Key: a.) fasciculus cuneatus b.) fasciculus gracilis c.) lateral corticospinal tract d.) anterior corticospinal tract d.) anterior corticosp..
The threat of revocation plays an important role : The threat of revocation plays an important role in the effective management of many offenders under community supervision. Describe revocation and discuss how it can be used as a tool to encourage compliance with the conditions of a community ..
Established the rickettsiae as bacteria rather than viruses : Identify what types of characteristics established the rickettsiae as bacteria rather than viruses. baced o nthe description of viruses, provide agruments for and against the statement, "viruses are living organisms".
Cloned dna sequence was used to probe a southern blot : A cloned DNA sequence was used to probe a Southern blot. There were 2 DNA samples on the blot, one from white blood cells and the other from a liver biopsy of the same individual. Both samples had been digested with HpaII. The probe bound to a sin..
What phenotypes and in what proportions will be produced : Color of fruits in summer squash is determined by two genes. Allele Y (dominant) of the first gene determines the yellow color, while alleley (recessive) determines the green color. These two colors can develop only if a recessive allelew of the s..
Obtain the speed of the can just before it hits the ground : A painter is standing on scaffolding that is raised at constant speed. As he travels upward, he accidentally nudges a paint can off the scaffolding and it falls a distance 15.4m to the ground. find the speed of the can just before it hits the groun..
Define what are some of these isomers and in a complex ion : Empirical formula Pt(NH3)2Cl3. Sketch at least four isomers which correspond with the given empirical formula and have a molecular mass of 670 g/mol. What are some of these isomers and in a complex ion, can a chlorine atom be bonded to two other a..
What is the speed of the truck when they are abreast : An automobile and a truck start from rest at the same instant, with the automobile initially at some distance behind the truck.  What is the speed of the truck when they are abreast

Reviews

Write a Review

Biology Questions & Answers

  How are somatic cells able to replicate its dna

How are somatic cells able to replicate its DNA and how would you identify the stages a cell cycle is undergoing?

  What is the amplitude of an earthquake

How do you find the concentration of a solution when given the info that the concentration of the stock solution is 1:57 and has a concentration of 5mg/ml.

  How can the following solutions be prepared

How can the following solutions be prepared?

  Decscribe how antigen presenting cells process

Decscribe how antigen presenting cells process antigens and presentantigen fragments in association with MHC class I proteins.

  Describe the changes in her total body water

Describe the changes in her total body water, intracellular fluid, plasma, and interstitial fluid levels that took place as a result of the pain, redness, and local swelling from the bee sting.

  How various real map units are indicated

If you obtain an RF value of 45 percent in one experiment, what can you say about linkage? (The actual figures are 58, 52, 47, and 43 out of 200 progeny)

  The overall reaction of aerobic respiration of glucose

The overall reaction of aerobic respiration of glucose is summarized as.

  Which structure is found in all prokaryotic cells

Which structure is found in ALL prokaryotic cells.

  Preparing new mating experiment with the strains

You have an F- strain of E. coli that is resistant to streptomycin but requires the following amino acids for growth on minimal medium: arginine, cysteine, methionine, phenylalanine, and proline.

  Hydrolases are one important class of enzyme

Hydrolases are one important class of enzyme that function to catalyze.

  Which structure found in angiosperms

Angiosperms are more advanced than gymnosperms because gymnosperms lack which structure found in angiosperms?

  Illustrate the difference between the prokaryotic and

question 1.what is the difference between prokaryotic and eukaryotic cells? what components do all cells have in

Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd