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Clone the insert dna into the vector plasmid

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Reference no: EM132486158

You are trying to clone the insert DNA into the vector plasmid. Here are the materials you were given:

  • Plasmid DNA (concentration = 150 ng/µl)
  • pBR322
  • pBLU
  • pAMP
  • Insert DNA (concentrations = 5 ng/µl)
  • kan gene (kanamycin resistance gene)
  • cat gene (chloramphenicol resistance gene)

If growth is observed on LA and LA + ampicillin plates, but not on either LA + chloramphenicol or LA + kanamycin, what step(s) might have failed? How could you verify this? Assume some ligation reaction volume was left over.

Reference no: EM132486158

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