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The citric acid cycle enzyme, fumarase, catalyzes the conversion of fumarate to malate:
Fumarate + H2O à Malate
The turnover number, kcat, for fumarase is 800/sec. The Km of fumarase for its substrate is 5uM.
(a) In an experiment using 2 nM of fumarase, what is Vmax?
(b) The cellular concentration of fumarate is 47.5 uM. What is V0 when [fumarate] = 47.5 uM?
(c) What is the catalytic efficiency of fumarase?
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