Chromatogram that gave retention times

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Problem- A student was asked to separate two substances, A and B, on a 30 cm column. She obtained a chromatogram that gave retention times of 15.80 and 17.23 min for A and B, respectively. The base peak widths for A and B were 1.25 and 1.38 min, respectively.

A) Average Number of Theoretical Plates

B) Plate Height

C) Resolution

D) What should be a length of the column to achieve resolution of 2? Hint: Resolution is directly proportional, but not equal, to the square root of column length

Mention all the steps and procedure to determine the correct answer

Reference no: EM13699820

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